Genome-wide Escherichia coli stress response and improved tolerance towards industrially relevant chemicals.

ABSTRACT:

Background

Economically viable biobased production of bulk chemicals and biofuels typically requires high product titers. During microbial bioconversion this often leads to product toxicity, and tolerance is therefore a critical element in the engineering of production strains.

Results

Here, a systems biology approach was employed to understand the chemical stress response of Escherichia coli, including a genome-wide screen for mutants with increased fitness during chemical stress. Twelve chemicals with significant production potential were selected, consisting of organic solvent-like chemicals (butanol, hydroxy-γ-butyrolactone, 1,4-butanediol, furfural), organic acids (acetate, itaconic acid, levulinic acid, succinic acid), amino acids (serine, threonine) and membrane-inter

SUBMITTER: Rau MH 

PROVIDER: S-EPMC5064937 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature