High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties.

ABSTRACT:

Background

Microbial synthesis of oleochemicals derived from native fatty acid (FA) metabolism has presented significant advances in recent years. Even so, native FA biosynthetic pathways often provide a narrow variety of usually linear hydrocarbons, thus yielding end products with limited structural diversity. To overcome this limitation, we took advantage of a polyketide synthase-based system from Mycobacterium tuberculosis and developed an Escherichia coli platform with the capacity to synthesize multimethyl-branched long-chain esters (MBE) with novel chemical structures.

Results

With the aim to initiate the characterization of these novel waxy compounds, here, we describe the chassis optimization of the MBE producer E. coli strain for an up-scaled oi

SUBMITTER: Menendez-Bravo S 

PROVIDER: S-EPMC5064953 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature