Nuclear pore assembly proceeds by an inside-out extrusion of the nuclear envelope.

ABSTRACT: The nuclear pore complex (NPC) mediates nucleocytoplasmic transport through the nuclear envelope. How the NPC assembles into this double membrane boundary has remained enigmatic. Here, we captured temporally staged assembly intermediates by correlating live cell imaging with high-resolution electron tomography and super-resolution microscopy. Intermediates were dome-shaped evaginations of the inner nuclear membrane (INM), that grew in diameter and depth until they fused with the flat outer nuclear membrane. Live and super-resolved fluorescence microscopy revealed the molecular maturation of the intermediates, which initially contained the nuclear and cytoplasmic ring component Nup107, and only later the cytoplasmic filament component Nup358. EM particle averaging showed that the evaginatio

SUBMITTER: Otsuka S 

PROVIDER: S-EPMC5065316 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature