Adoptive Transfer of Tracer-Alloreactive CD4⁺ T Cell Receptor Transgenic T Cells Alters the Endogenous Immune Response to an Allograft.

ABSTRACT: T cell receptor transgenic (TCR-Tg) T cells are often used as tracer populations of antigen-specific responses to extrapolate findings to endogenous T cells. The extent to which TCR-Tg T cells behave purely as tracer cells or modify the endogenous immune response is not clear. To test the impact of TCR-Tg T cell transfer on endogenous alloimmunity, recipient mice were seeded with CD4⁺ or CD8⁺ TCR-Tg or polyclonal T cells at the time of cardiac allograft transplantation. Only CD4⁺ TCR-Tg T cells accelerated rejection and, unexpectedly, led to a dose-dependent decrease in both transferred and endogenous T cells infiltrating the graft. In contrast, recipients of CD4⁺ TCR-Tg T cells exhibited enhanced endogenous donor-specific CD8⁺ T cell

SUBMITTER: Miller ML 

PROVIDER: S-EPMC5065388 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature