Transdifferentiation Requires iNOS Activation: Role of RING1A S-Nitrosylation.

ABSTRACT:

Rationale

We have previously shown that innate immunity is necessary for transdifferentiation of fibroblasts to endothelial cells. A major signaling molecule involved in innate immunity is inducible nitric oxide synthase (iNOS). Accordingly, we hypothesized that iNOS-generated nitric oxide (NO) might enhance transdifferentiation.

Objective

To elucidate the role of NO in epigenetic plasticity during transdifferentiation.

Methods and results

We exposed the BJ fibroblasts to transdifferentiation formulation that included endothelial growth factors and innate immune activator polyinosinic:polycytidylic acid to induce endothelial cells. Generation of transdifferentiated endothelial cells was associated with iNOS expression and NO elaboration. In the absence of polyinosinic

SUBMITTER: Meng S 

PROVIDER: S-EPMC5065398 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature