N-glycosylation proteome enrichment analysis in kidney reveals differences between diabetic mouse models.

ABSTRACT:

Background

Diabetic nephropathy (DN) is a late complication in both type 1 diabetes mellitus (T1DM) and T2DM. Already at an early stage of DN morphological changes occur at the cell surface and in the extracellular matrix where the majority of the proteins carry N-linked glycosylations. These glycosylated proteins are highly important in cell adhesion and cell-matrix processes but not much is known about how they change in DN or whether the distinct etiology of T1DM and T2DM could have an effect on their abundances.

Method

We enriched for the N-glycosylated kidney proteome in db/db mice dosed with insulin or vehicle, in streptozotocin-induced (STZ) diabetic mice and healthy control mice dosed with vehicle. Glycopeptides were analyzed with label-free shotgun mass spectrometry

SUBMITTER: Liljedahl L 

PROVIDER: S-EPMC5065702 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature