Multiple Natural and Experimental Inflammatory Rabbit Lacrimal Gland Phenotypes.

ABSTRACT:

Purpose

To investigate lacrimal gland (LG) immunophysiological and immune-mediated inflammatory process (IMIP) phenotype diversity.

Methods

Ex vivo matured dendritic cells (mDC) were loaded with acinar cell microparticles (M_P). Peripheral blood lymphocytes (PBL) were activated in mixed cell reactions with mDC and injected directly into autologous, unilateral LG (1°ATD-LG) of two rabbit cohorts, one naïve, one immunized with a LG lysate membrane fraction (P_i). Autoimmune IgG titers were assayed by ELISA, MCR PBL stimulation indices (SI) by [³H]-thymidine incorporation. Schirmer tests without and with topical anesthetic (STT-I, STT-I_A) and rose Bengal (RB) staining tests were performed. H&E and immunohistochemically stained sections

SUBMITTER: Mircheff AK 

PROVIDER: S-EPMC5065763 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature