Different next generation sequencing platforms produce different microbial profiles and diversity in cystic fibrosis sputum.

ABSTRACT:

Background

Cystic fibrosis (CF) is an autosomal recessive disease characterized by recurrent lung infections. Studies of the lung microbiome have shown an association between decreasing diversity and progressive disease. 454 pyrosequencing has frequently been used to study the lung microbiome in CF, but will no longer be supported. We sought to identify the benefits and drawbacks of using two state-of-the-art next generation sequencing (NGS) platforms, MiSeq and PacBio RSII, to characterize the CF lung microbiome. Each has its advantages and limitations.

Methods

Twelve samples of extracted bacterial DNA were sequenced on both MiSeq and PacBio NGS platforms. DNA was amplified for the V4 region of the 16S rRNA gene and libraries were sequenced on the MiSeq sequencing platform,

SUBMITTER: Hahn A 

PROVIDER: S-EPMC5065784 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature