Core/shell protein-reactive nanogels via a combination of RAFT polymerization and vinyl sulfone postmodification.

ABSTRACT:

Aim

A promising nanogel vaccine platform was expanded toward antigen conjugation.

Materials & methods

Block copolymers containing a reactive ester solvophobic block and a PEG-like solvophilic block were synthesized via reversible addition-fragmentation chain-transfer polymerization. Following self-assembly in DMSO, the esters allow for core-crosslinking and hydrophilization by amide bond formation with primary amines. Free thiols were accessed at the polymer chain ends through aminolysis of the reversible addition-fragmentation chain-transfer groups, and into the nanogel core by reactive ester conversion with cysteamine. Subsequently, free thiols were converted into vinyl sulfone moieties.

Results

Despite sterical constraints, nanogel-associated vinyl sulfone moieties

SUBMITTER: Vanparijs N 

PROVIDER: S-EPMC5066120 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature