Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A.

ABSTRACT: The chromokinesin KIF4A has been implicated in shaping mitotic chromosomes, but its functional relationship to condensin complexes remains controversial. Here, we found that, in mitosis, KIF4A associates with condensin I but not with condensin II. Mutational analyses indicated that the enrichment of condensin I to chromosomal axes depends on its association with KIF4A in a way that likely involves its motor activity. Remarkably, this interaction is required for condensin I to confer physiological properties to chromosomes. These observations provide an insight into how condensin I is enriched at chromosomal axes and underscore the significance of axial structure in organizing mitotic chromosomes.

SUBMITTER: Takahashi M 

PROVIDER: S-EPMC5066236 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature