Dynamics of the mouse brain cortical synaptic proteome during postnatal brain development.

ABSTRACT: Development of the brain involves the formation and maturation of numerous synapses. This process requires prominent changes of the synaptic proteome and potentially involves thousands of different proteins at every synapse. To date the proteome analysis of synapse development has been studied sparsely. Here, we analyzed the cortical synaptic membrane proteome of juvenile postnatal days 9 (P9), P15, P21, P27, adolescent (P35) and different adult ages P70, P140 and P280 of C57Bl6/J mice. Using a quantitative proteomics workflow we quantified 1560 proteins of which 696 showed statistically significant differences over time. Synaptic proteins generally showed increased levels during maturation, whereas proteins involved in protein synthesis generally decreased in abundance. In several cases,

SUBMITTER: Gonzalez-Lozano MA 

PROVIDER: S-EPMC5066275 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature