A simple and efficient method to visualize and quantify the efficiency of chromosomal mutations from genome editing.

ABSTRACT: Genome editing with designer nucleases such as TALEN and CRISPR/Cas enzymes has broad applications. Delivery of these designer nucleases into organisms induces various genetic mutations including deletions, insertions and nucleotide substitutions. Characterizing those mutations is critical for evaluating the efficacy and specificity of targeted genome editing. While a number of methods have been developed to identify the mutations, none other than sequencing allows the identification of the most desired mutations, i.e., out-of-frame insertions/deletions that disrupt genes. Here we report a simple and efficient method to visualize and quantify the efficiency of genomic mutations induced by genome-editing. Our approach is based on the expression of a two-color fusion protein in a vector that

SUBMITTER: Fu L 

PROVIDER: S-EPMC5066342 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature