Role of Phosphate Transport System Component PstB1 in Phosphate Internalization by Nostoc punctiforme.

ABSTRACT: In bacteria, limited phosphate availability promotes the synthesis of active uptake systems, such as the Pst phosphate transport system. To understand the mechanisms that facilitate phosphate accumulation in the cyanobacterium Nostoc punctiforme, phosphate transport systems were identified, revealing a redundancy of Pst phosphate uptake systems that exists across three distinct operons. Four separate PstB system components were identified. pstB1 was determined to be a suitable target for creating phenotypic mutations that could result in the accumulation of excessive levels of phosphate through its overexpression or in a reduction of the capacity to accumulate phosphate through its deletion. Using quantitative real-time PCR (qPCR), it was determined that pstB1 mRNA levels increased signifi

SUBMITTER: Hudek L 

PROVIDER: S-EPMC5066351 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature