Mechanistic insight into eukaryotic 60S ribosomal subunit biogenesis by cryo-electron microscopy.

ABSTRACT: Eukaryotic ribosomes, the protein-producing factories of the cell, are composed of four ribosomal RNA molecules and roughly 80 proteins. Their biogenesis is a complex process that involves more than 200 biogenesis factors that facilitate the production, modification, and assembly of ribosomal components and the structural transitions along the maturation pathways of the pre-ribosomal particles. Here, I review recent structural and mechanistic insights into the biogenesis of the large ribosomal subunit that were furthered by cryo-electron microscopy of natively purified pre-60S particles and in vitro reconstituted ribosome assembly factor complexes. Combined with biochemical, genetic, and previous structural data, these structures have provided detailed insights into the assembly and matura

SUBMITTER: Greber BJ 

PROVIDER: S-EPMC5066618 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature