An intermolecular G-quadruplex as the basis for GTP recognition in the class V-GTP aptamer.

ABSTRACT: Many naturally occurring or artificially created RNAs are capable of binding to guanine or guanine derivatives with high affinity and selectivity. They bind their ligands using very different recognition modes involving a diverse set of hydrogen bonding and stacking interactions. Apparently, the potential structural diversity for guanine, guanosine, and guanine nucleotide binding motifs is far from being fully explored. Szostak and coworkers have derived a large set of different GTP-binding aptamer families differing widely in sequence, secondary structure, and ligand specificity. The so-called class V-GTP aptamer from this set binds GTP with very high affinity and has a complex secondary structure. Here we use solution NMR spectroscopy to demonstrate that the class V aptamer binds GTP thr

SUBMITTER: Nasiri AH 

PROVIDER: S-EPMC5066627 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature