TRNA base methylation identification and quantification via high-throughput sequencing.

ABSTRACT: Eukaryotic transfer RNAs contain on average 14 modifications. Investigations of their biological functions require the determination of the modification sites and the dynamic variations of the modification fraction. Base methylation represents a major class of tRNA modification. Although many approaches have been used to identify tRNA base methylations, including sequencing, they are generally qualitative and do not report the information on the modification fraction. Dynamic mRNA modifications have been shown to play important biological roles; yet, the extent of tRNA modification fractions has not been reported systemically. Here we take advantage of a recently developed high-throughput sequencing method (DM-tRNA-seq) to identify and quantify tRNA base methylations located at the Watson-

SUBMITTER: Clark WC 

PROVIDER: S-EPMC5066629 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature