BioNano genome mapping of individual chromosomes supports physical mapping and sequence assembly in complex plant genomes.

ABSTRACT: The assembly of a reference genome sequence of bread wheat is challenging due to its specific features such as the genome size of 17 Gbp, polyploid nature and prevalence of repetitive sequences. BAC-by-BAC sequencing based on chromosomal physical maps, adopted by the International Wheat Genome Sequencing Consortium as the key strategy, reduces problems caused by the genome complexity and polyploidy, but the repeat content still hampers the sequence assembly. Availability of a high-resolution genomic map to guide sequence scaffolding and validate physical map and sequence assemblies would be highly beneficial to obtaining an accurate and complete genome sequence. Here, we chose the short arm of chromosome 7D (7DS) as a model to demonstrate for the first time that it is possible to couple ch

SUBMITTER: Stankova H 

PROVIDER: S-EPMC5066648 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature