Simultaneous multiview capture and fusion improves spatial resolution in wide-field and light-sheet microscopy.

ABSTRACT: Most fluorescence microscopes are inefficient, collecting only a small fraction of the emitted light at any instant. Besides wasting valuable signal, this inefficiency also reduces spatial resolution and causes imaging volumes to exhibit significant resolution anisotropy. We describe microscopic and computational techniques that address these problems by simultaneously capturing and subsequently fusing and deconvolving multiple specimen views. Unlike previous methods that serially capture multiple views, our approach improves spatial resolution without introducing any additional illumination dose or compromising temporal resolution relative to conventional imaging. When applying our methods to single-view wide-field or dual-view light-sheet microscopy, we achieve a twofold improvement in v

SUBMITTER: Wu Y 

PROVIDER: S-EPMC5066810 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature