Improved Peak Detection and Deconvolution of Native Electrospray Mass Spectra from Large Protein Complexes.

ABSTRACT: Native electrospray-ionization mass spectrometry (native MS) measures biomolecules under conditions that preserve most aspects of protein tertiary and quaternary structure, enabling direct characterization of large intact protein assemblies. However, native spectra derived from these assemblies are often partially obscured by low signal-to-noise as well as broad peak shapes because of residual solvation and adduction after the electrospray process. The wide peak widths together with the fact that sequential charge state series from highly charged ions are closely spaced means that native spectra containing multiple species often suffer from high degrees of peak overlap or else contain highly interleaved charge envelopes. This situation presents a challenge for peak detection, correct charg

SUBMITTER: Lu J 

PROVIDER: S-EPMC5067139 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature