Enhanced production of polyunsaturated fatty acids by enzyme engineering of tandem acyl carrier proteins.

ABSTRACT: In some microorganisms, polyunsaturated fatty acids (PUFAs) are biosynthesized by PUFA synthases characterized by tandem acyl carrier proteins (ACPs) in subunit A. These ACPs were previously shown to be important for PUFA productivity. In this study, we examined their function in more detail. PUFA productivities increased depending on the number of ACPs without profile changes in each subunit A of eukaryotic and prokaryotic PUFA synthases. We also constructed derivative enzymes from subunit A with 5 × ACPs. Enzymes possessing one inactive ACP at any position produced ~30% PUFAs compared with the parental enzyme but unexpectedly had ~250% productivity compared with subunit A with 4 × ACPs. Enzymes constructed by replacing the 3^rd ACP with an inactive ACP from another subunit A or

SUBMITTER: Hayashi S 

PROVIDER: S-EPMC5067506 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature