A rapid co-culture stamping device for studying intercellular communication.

ABSTRACT: Regulation of tissue development and repair depends on communication between neighbouring cells. Recent advances in cell micro-contact printing and microfluidics have facilitated the in-vitro study of homotypic and heterotypic cell-cell interaction. Nonetheless, these techniques are still complicated to perform and as a result, are seldom used by biologists. We report here development of a temporarily sealed microfluidic stamping device which utilizes a novel valve design for patterning two adherent cell lines with well-defined interlacing configurations to study cell-cell interactions. We demonstrate post-stamping cell viability of >95%, the stamping of multiple adherent cell types, and the ability to control the seeded cell density. We also show viability, proliferation and migration of

SUBMITTER: Hassanzadeh-Barforoushi A 

PROVIDER: S-EPMC5067516 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature