Bean pod mottle virus: a new powerful tool for functional genomics studies in Pisum sativum.

ABSTRACT: Pea (Pisum sativum L.) is an important legume worldwide. The importance of pea in arable rotations and nutritional value for both human and animal consumption have fostered sustained production and different studies to improve agronomic traits of interest. Moreover, complete sequencing of the pea genome is currently underway and will lead to the identification of a large number of genes potentially associated with important agronomic traits. Because stable genetic transformation is laborious for pea, virus-induced gene silencing (VIGS) appears as a powerful alternative technology for determining the function of unknown genes. In this work, we present a rapid and efficient viral inoculation method using DNA infectious plasmids of Bean pod mottle virus (BPMV)-derived VIGS vector. Six pea gen

SUBMITTER: Meziadi C 

PROVIDER: S-EPMC5067550 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature