F-O-G Ring Formation in Glycopeptide Antibiotic Biosynthesis is Catalysed by OxyE.

ABSTRACT: The glycopeptide antibiotics are peptide-based natural products with impressive antibiotic function that derives from their unique three-dimensional structure. Biosynthesis of the glycopeptide antibiotics centres of the combination of peptide synthesis, mediated by a non-ribosomal peptide synthetase, and the crosslinking of aromatic side chains of the peptide, mediated by the action of a cascade of Cytochrome P450s. Here, we report the first example of in vitro activity of OxyE, which catalyses the F-O-G ring formation reaction in teicoplanin biosynthesis. OxyE was found to only act after an initial C-O-D crosslink is installed by OxyB and to require an interaction with the unique NRPS domain from glycopeptide antibiotic - the X-domain - in order to display catalytic activity. We could dem

SUBMITTER: Peschke M 

PROVIDER: S-EPMC5067714 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature