Bacterial Populations Associated with Smokeless Tobacco Products.

ABSTRACT: There are an estimated 8 million users of smokeless tobacco products (STPs) in the United States, and yet limited data on microbial populations within these products exist. To better understand the potential microbiological risks associated with STP use, a study was conducted to provide a baseline microbiological profile of STPs. A total of 90 samples, representing 15 common STPs, were purchased in metropolitan areas in Little Rock, AR, and Washington, DC, in November 2012, March 2013, and July 2013. Bacterial populations were evaluated using culture, pyrosequencing, and denaturing gradient gel electrophoresis (DGGE). Moist-snuff products exhibited higher levels of bacteria (average of 1.05 × 10⁶ CFU/g STP) and diversity of bacterial populations than snus (average of 8.33 × 10

SUBMITTER: Han J 

PROVIDER: S-EPMC5068160 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature