Quantitative imaging for development of companion diagnostics to drugs targeting HGF/MET.

ABSTRACT: The limited clinical success of anti-HGF/MET drugs can be attributed to the lack of predictive biomarkers that adequately select patients for treatment. We demonstrate here that quantitative digital imaging of formalin fixed paraffin embedded tissues stained by immunohistochemistry can be used to measure signals from weakly staining antibodies and provides new opportunities to develop assays for detection of MET receptor activity. To establish a biomarker panel of MET activation, we employed seven antibodies measuring protein expression in the HGF/MET pathway in 20 cases and up to 80 cores from 18 human cancer types. The antibodies bind to epitopes in the extra (EC)- and intracellular (IC) domains of MET (MET4^EC, SP44_MET^IC, D1C2_MET^IC), to MET-pY1234/pY123

SUBMITTER: Huang F 

PROVIDER: S-EPMC5068192 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature