New separation-free assay technique for SNPs using two-photon excitation fluorometry.

ABSTRACT: A new separation-free method for detection of single nucleotide polymorphisms (SNPs) is described. The method is based on the single base extension principle, fluorescently labeled dideoxy nucleotides and two-photon fluorescence excitation technology, known as ArcDia trade mark TPX technology. In this assay technique, template-directed single base extension is carried out for primers which have been immobilized on polymer microparticles. Depending on the sequence of the template DNA, the primers are extended either with a labeled or with a non-labeled nucleotide. The genotype of the sample is determined on the basis of two-photon excited fluorescence of individual microparticles. The effect of various assay condition parameters on the performance of the assay method is studied. The perform

SUBMITTER: Vaarno J 

PROVIDER: S-EPMC506825 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature