Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by disulfide reductase ERdj5.

ABSTRACT: Calcium ion (Ca²⁺) is an important second messenger that regulates numerous cellular functions. Intracellular Ca²⁺ concentration ([Ca²⁺]i) is strictly controlled by Ca²⁺ channels and pumps on the endoplasmic reticulum (ER) and plasma membranes. The ER calcium pump, sarco/endoplasmic reticulum calcium ATPase (SERCA), imports Ca²⁺ from the cytosol into the ER in an ATPase activity-dependent manner. The activity of SERCA2b, the ubiquitous isoform of SERCA, is negatively regulated by disulfide bond formation between two luminal cysteines. Here, we show that ERdj5, a mammalian ER disulfide reductase, which we reported to be involved in the ER-associated degradation of misfolded proteins, activates the pump function of SERCA2b by reducing it

SUBMITTER: Ushioda R 

PROVIDER: S-EPMC5068290 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature