Functional receptor molecules CD300lf and CD300ld within the CD300 family enable murine noroviruses to infect cells.

ABSTRACT: Norovirus is the leading cause of acute gastroenteritis worldwide. Since the discovery of human norovirus (HuNoV), an efficient and reproducible norovirus replication system has not been established in cultured cells. Although limited amounts of virus particles can be produced when the HuNoV genome is directly transfected into cells, the HuNoV cycle of infection has not been successfully reproduced in any currently available cell-culture system. Those results imply that the identification of a functional cell-surface receptor for norovirus might be the key to establishing a norovirus culture system. Using a genome-wide CRISPR/Cas9 guide RNA library, we identified murine CD300lf and CD300ld as functional receptors for murine norovirus (MNV). The treatment of susceptible cells with polyclona

SUBMITTER: Haga K 

PROVIDER: S-EPMC5068309 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature