Identification and temporal expression of putative circadian clock transcripts in the amphipod crustacean Talitrus saltator.

ABSTRACT:

Background

Talitrus saltator is an amphipod crustacean that inhabits the supralittoral zone on sandy beaches in the Northeast Atlantic and Mediterranean. T. saltator exhibits endogenous locomotor activity rhythms and time-compensated sun and moon orientation, both of which necessitate at least one chronometric mechanism. Whilst their behaviour is well studied, currently there are no descriptions of the underlying molecular components of a biological clock in this animal, and very few in other crustacean species.

Methods

We harvested brain tissue from animals expressing robust circadian activity rhythms and used homology cloning and Illumina RNAseq approaches to sequence and identify the core circadian clock and clock-related genes in these samples. We assessed

SUBMITTER: O'Grady JF 

PROVIDER: S-EPMC5068443 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature