Efficiency in Complexity: Composition and Dynamic Nature of Mimivirus Replication Factories.

ABSTRACT: The recent discovery of multiple giant double-stranded DNA (dsDNA) viruses blurred the consensual distinction between viruses and cells due to their size, as well as to their structural and genetic complexity. A dramatic feature revealed by these viruses as well as by many positive-strand RNA viruses is their ability to rapidly form elaborate intracellular organelles, termed "viral factories," where viral progeny are continuously generated. Here we report the first isolation of viral factories at progressive postinfection time points. The isolated factories were subjected to mass spectrometry-based proteomics, bioinformatics, and imaging analyses. These analyses revealed that numerous viral proteins are present in the factories but not in mature virions, thus implying that multiple and div

SUBMITTER: Fridmann-Sirkis Y 

PROVIDER: S-EPMC5068508 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature