Estimating the Effective Population Size from Temporal Allele Frequency Changes in Experimental Evolution.

ABSTRACT: The effective population size ([Formula: see text]) is a major factor determining allele frequency changes in natural and experimental populations. Temporal methods provide a powerful and simple approach to estimate short-term [Formula: see text] They use allele frequency shifts between temporal samples to calculate the standardized variance, which is directly related to [Formula: see text] Here we focus on experimental evolution studies that often rely on repeated sequencing of samples in pools (Pool-seq). Pool-seq is cost-effective and often outperforms individual-based sequencing in estimating allele frequencies, but it is associated with atypical sampling properties: Additional to sampling individuals, sequencing DNA in pools leads to a second round of sampling, which increases the var

SUBMITTER: Jonas A 

PROVIDER: S-EPMC5068858 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature