Interallelic Transcriptional Enhancement as an in Vivo Measure of Transvection in Drosophila melanogaster.

ABSTRACT: Transvection-pairing-dependent interallelic regulation resulting from enhancer action in trans-occurs throughout the Drosophila melanogaster genome, likely as a result of the extensive somatic homolog pairing seen in Dipteran species. Recent studies of transvection in Drosophila have demonstrated important qualitative differences between enhancer action in cis vs. in trans, as well as a modest synergistic effect of cis- and trans-acting enhancers on total tissue transcript levels at a given locus. In the present study, we identify a system in which cis- and trans-acting GAL4-UAS enhancer synergism has an unexpectedly large quantitative influence on gene expression, boosting total tissue transcript levels at least fourfold relative to those seen in the absence of transvection. We exploit th

SUBMITTER: Noble GP 

PROVIDER: S-EPMC5068936 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature