Lactate metabolism is associated with mammalian mitochondria.

ABSTRACT: It is well established that lactate secreted by fermenting cells can be oxidized or used as a gluconeogenic substrate by other cells and tissues. It is generally assumed, however, that within the fermenting cell itself, lactate is produced to replenish NAD⁺ and then is secreted. Here we explore the possibility that cytosolic lactate is metabolized by the mitochondria of fermenting mammalian cells. We found that fermenting HeLa and H460 cells utilize exogenous lactate carbon to synthesize a large percentage of their lipids. Using high-resolution mass spectrometry, we found that both ¹³C and 2-²H labels from enriched lactate enter the mitochondria. The lactate dehydrogenase (LDH) inhibitor oxamate decreased respiration of isolated mitochondria incubated in la

SUBMITTER: Chen YJ 

PROVIDER: S-EPMC5069139 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature