A rare subset of skin-tropic regulatory T cells expressing Il10/Gzmb inhibits the cutaneous immune response.

ABSTRACT: Foxp3⁺ regulatory T cells (Tregs) migrating from the skin to the draining lymph node (dLN) have a strong immunosuppressive effect on the cutaneous immune response. However, the subpopulations responsible for their inhibitory function remain unclear. We investigated single-cell gene expression heterogeneity in Tregs from the dLN of inflamed skin in a contact hypersensitivity model. The immunosuppressive genes Ctla4 and Tgfb1 were expressed in the majority of Tregs. Although Il10-expressing Tregs were rare, unexpectedly, the majority of Il10-expressing Tregs co-expressed Gzmb and displayed Th1-skewing. Single-cell profiling revealed that CD43⁺ CCR5⁺ Tregs represented the main subset within the Il10/Gzmb-expressing cell population in the dLN. Moreover, CD43

SUBMITTER: Ikebuchi R 

PROVIDER: S-EPMC5069467 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature