Discovery of putative capsaicin biosynthetic genes by RNA-Seq and digital gene expression analysis of pepper.

ABSTRACT: The Indian pepper 'Guijiangwang' (Capsicum frutescens L.), one of the world's hottest chili peppers, is rich in capsaicinoids. The accumulation of the alkaloid capsaicin and its analogs in the epidermal cells of the placenta contribute to the pungency of Capsicum fruits. To identify putative genes involved in capsaicin biosynthesis, RNA-Seq was used to analyze the pepper's expression profiles over five developmental stages. Five cDNA libraries were constructed from the total RNA of placental tissue and sequenced using an Illumina HiSeq 2000. More than 19 million clean reads were obtained from each library, and greater than 50% of the reads were assignable to reference genes. Digital gene expression (DGE) profile analysis using Solexa sequencing was performed at five fruit developmental sta

SUBMITTER: Zhang ZX 

PROVIDER: S-EPMC5069471 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature