Ontology highlight
ABSTRACT:
SUBMITTER: Baslan T
PROVIDER: S-EPMC5069701 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Nature protocols 20120503 6
Copy number variation (CNV) is increasingly recognized as an important contributor to phenotypic variation in health and disease. Most methods for determining CNV rely on admixtures of cells in which information regarding genetic heterogeneity is lost. Here we present a protocol that allows for the genome-wide copy number analysis of single nuclei isolated from mixed populations of cells. Single-nucleus sequencing (SNS), combines flow sorting of single nuclei on the basis of DNA content and whol ...[more]