Characterization of the Δ7 Mutant of Cupriavidus metallidurans with Deletions of Seven Secondary Metal Uptake Systems.

ABSTRACT: Central to the ability of Cupriavidus metallidurans to maintain its metal homoeostasis is the metal transportome, composed of uptake and efflux systems. Seven secondary metal import systems, ZupT, PitA, CorA₁, CorA₂, CorA₃, ZntB, and HoxN, interact and are at the core of the metal uptake transportome. The 7-fold deletion mutant Δ7 (ΔzupT ΔpitA ΔcorA₁ΔcorA₂ΔcorA₃ΔzntB ΔhoxN) of parent strain AE104 is still able to maintain its cellular metal content, although at the cost of reduced fitness (M. Herzberg, L. Bauer, A. Kirsten, and D. H. Nies, Metallomics, in press, http://dx.doi.org/10.1039/C5MT00295H). Strain Δ7 does not express genes for backup importers, and so Δ7 should use metal uptake sy

SUBMITTER: Große C 

PROVIDER: S-EPMC5069749 | biostudies-literature | 2016 Jan-Feb

REPOSITORIES: biostudies-literature