Comprehensive investigations of biobutanol production by a non-acetone and 1,3-propanediol generating Clostridium strain from glycerol and polysaccharides.

ABSTRACT:

Background

Low-cost feedstocks, a single product (butanol), and a high butanol titer are three key points for establishing a sustainable and economically viable process for biological butanol production. Here, we comprehensively investigated the butanol production from mono-substrates, mainly glycerol and polysaccharides, mainly starch and xylan by a newly identified wild-type Clostridium pasteurianum GL11.

Results

Strain GL11 produced 14.7 g/L of butanol with a yield of 0.41 g/g from glycerol in the batch mode without formation of by-products of acetone and 1,3-propanediol (1,3-PDO). With in situ extraction with biodiesel, the amount of butanol was finally improved to 28.8 g/L in the fed-batch mode. Genomic and enzymatic analysis showed that the deficiency of key enz

SUBMITTER: Xin F 

PROVIDER: S-EPMC5069857 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature