Integrins are not essential for entry of coxsackievirus A9 into SW480 human colon adenocarcinoma cells.

ABSTRACT:

Background

Coxsackievirus A9 (CV-A9) is a pathogenic enterovirus type within the family Picornaviridae. CV-A9 infects A549 human epithelial lung carcinoma cells by attaching to the αVβ6 integrin receptor through a highly conserved Arg-Gly-Asp (RGD) motif, which is located at the exposed carboxy-terminus of the capsid protein VP1 detected in all studied clinical isolates. However, genetically-modified CV-A9 that lacks the RGD motif (CV-A9-RGDdel) has been shown to be infectious in some cell lines but not in A549, suggesting that RGD-mediated integrin binding is not always essential for efficient entry of CV-A9.

Methods

Two cell lines, A549 and SW480, were used in the study. SW480 was the study object for the integrin-independent entry and A549 was used as the control for inte

SUBMITTER: Heikkila O 

PROVIDER: S-EPMC5069866 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature