Proteome Remodeling in Response to Sulfur Limitation in "Candidatus Pelagibacter ubique".

ABSTRACT: The alphaproteobacterium "Candidatus Pelagibacter ubique" strain HTCC1062 and most other members of the SAR11 clade lack genes for assimilatory sulfate reduction, making them dependent on organosulfur compounds that occur naturally in seawater. To investigate how these cells adapt to sulfur limitation, batch cultures were grown in defined medium containing either limiting or nonlimiting amounts of dimethylsulfoniopropionate (DMSP) as the sole sulfur source. Protein and mRNA expression were measured before, during, and after the transition from exponential growth to stationary phase. Two distinct responses were observed, one as DMSP became exhausted and another as the cells acclimated to a sulfur-limited environment. The first response was characterized by increased transcription and

SUBMITTER: Smith DP 

PROVIDER: S-EPMC5069961 | biostudies-literature | 2016 Jul-Aug

REPOSITORIES: biostudies-literature