Formaldehyde Induces Rho-Associated Kinase Activity to Evoke Airway Hyperresponsiveness.

ABSTRACT: Formaldehyde, a common indoor air pollutant, exacerbates asthma and synergizes with allergen to induce airway hyperresponsiveness (AHR) in animal models. The mechanisms mediating formaldehyde-induced AHR remain poorly understood. We posit that formaldehyde modulates agonist-induced contractile response of human airway smooth muscle (HASM) cells to elicit AHR. HASM cells were exposed to formaldehyde or vehicle and agonist-induced intracellular Ca²⁺ ([Ca²⁺]_i) and myosin light-chain phosphatase (MYPT1) phosphorylation were determined. Air-liquid interface-differentiated human bronchial epithelial (HBE) cells were exposed to formaldehyde or vehicle and cocultured with HASM cells. Agonist-induced [Ca²⁺]_i and MYPT1 phosphorylation were deter

SUBMITTER: Jude J 

PROVIDER: S-EPMC5070102 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature