Gene expression profiling of trout regenerating muscle reveals common transcriptional signatures with hyperplastic growth zones of the post-embryonic myotome.

ABSTRACT:

Background

Muscle fibre hyperplasia stops in most fish when they reach approximately 50 % of their maximum body length. However, new small-diameter muscle fibres can be produced de novo in aged fish after muscle injury. Given that virtually nothing is known regarding the transcriptional mechanisms that regulate regenerative myogenesis in adult fish, we explored the temporal changes in gene expression during trout muscle regeneration following mechanical crushing. Then, we compared the gene transcription profiles of regenerating muscle with the previously reported gene expression signature associated with muscle fibre hyperplasia.

Results

Using an Agilent-based microarray platform we conducted a time-course analysis of transcript expression in 29 month-old trout muscle before

SUBMITTER: Montfort J 

PROVIDER: S-EPMC5070125 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature