Circulating granulysin levels in healthcare workers and latent tuberculosis infection estimated using interferon-gamma release assays.

ABSTRACT:

Background

Granulysin (GNLY) is produced by human lymphocyte subpopulations and exhibits antimicrobial activity against Mycobacterium tuberculosis. We examined the association between GNLY levels in blood and latent tuberculosis (TB) infection.

Methods

Latency of TB infection among Vietnamese healthcare workers was estimated using interferon-gamma release assays (IGRA), and serum GNLY concentrations were measured using enzyme-linked immunosorbent assays. The levels of GNLY expression in whole blood and the presence of GNLY alleles with the exon-4 polymorphism rs11127 were also determined using PCR-based methods.

Results

Among 109 study participants, 41 (37.6 %) were IGRA positive and had significantly lower serum GNLY concentrations compared with IGRA-negative partici

SUBMITTER: Thuong PH 

PROVIDER: S-EPMC5070182 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature