Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues.

ABSTRACT: The biology of multicellular organisms is coordinated across multiple size scales, from the subnanoscale of molecules to the macroscale, tissue-wide interconnectivity of cell populations. Here we introduce a method for super-resolution imaging of the multiscale organization of intact tissues. The method, called magnified analysis of the proteome (MAP), linearly expands entire organs fourfold while preserving their overall architecture and three-dimensional proteome organization. MAP is based on the observation that preventing crosslinking within and between endogenous proteins during hydrogel-tissue hybridization allows for natural expansion upon protein denaturation and dissociation. The expanded tissue preserves its protein content, its fine subcellular details, and its organ-scale inter

SUBMITTER: Ku T 

PROVIDER: S-EPMC5070610 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature