Phospho-Network Analysis Identifies and Quantifies Hepatitis C Virus (HCV)-induced Hepatocellular Carcinoma (HCC) Proteins Regulating Viral-mediated Tumor Growth.

ABSTRACT:

Background

Patients with chronic hepatitis C virus (HCV) infection are at risk of serious complications of cirrhosis and hepatocellular carcinoma (HCC). Mass spectrometry (MS) is a versatile methodology that produces a global proteomic landscape for analysis of cancer mechanisms.

Materials and methods

Using multiplex peptide stable isotopic labeling and immobilized metal affinity chromatography (IMAC), we enriched and quantified the phosphoproteome of HCC, with and without HCV. While raw data identified protein targets based on expression alone, we also used abundance groups for comprehensive functional analysis.

Results

Analysis of functional differences highlighted deregulated phosphoprotein networks. This uncovered additional candidates that could be directly deriv

SUBMITTER: Lu NT 

PROVIDER: S-EPMC5070624 | biostudies-literature | 2016 09-10

REPOSITORIES: biostudies-literature