Instrument for Real-Time Digital Nucleic Acid Amplification on Custom Microfluidic Devices.

ABSTRACT: Nucleic acid amplification tests that are coupled with a digital readout enable the absolute quantification of single molecules, even at ultralow concentrations. Digital methods are robust, versatile and compatible with many amplification chemistries including isothermal amplification, making them particularly invaluable to assays that require sensitive detection, such as the quantification of viral load in occult infections or detection of sparse amounts of DNA from forensic samples. A number of microfluidic platforms are being developed for carrying out digital amplification. However, the mechanistic investigation and optimization of digital assays has been limited by the lack of real-time kinetic information about which factors affect the digital efficiency and analytical sensitivity of

SUBMITTER: Selck DA 

PROVIDER: S-EPMC5070811 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature