Application of Targeted Mass Spectrometry for the Quantification of Sirtuins in the Central Nervous System.

ABSTRACT: Sirtuin proteins have a variety of intracellular targets, thereby regulating multiple biological pathways including neurodegeneration. However, relatively little is currently known about the role or expression of the 7 mammalian sirtuins in the central nervous system. Western blotting, PCR and ELISA are the main techniques currently used to measure sirtuin levels. To achieve sufficient sensitivity and selectivity in a multiplex-format, a targeted mass spectrometric assay was developed and validated for the quantification of all seven mammalian sirtuins (SIRT1-7). Quantification of all peptides was by multiple reaction monitoring (MRM) using three mass transitions per protein-specific peptide, two specific peptides for each sirtuin and a stable isotope labelled internal standard. The assay

SUBMITTER: Jayasena T 

PROVIDER: S-EPMC5071856 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature