Microfluidic co-culture system for cancer migratory analysis and anti-metastatic drugs screening.

ABSTRACT: Tumour metastasis is an important reason for cancer death, and cancer cell migration is an important step in the process of tumour metastasis. Studying cancer cell migration is of great significance. Here, we present a novel microfluidic co-culture system and establish mild, moderate and severe cancer models by using HMEpiC and MDA-MB-231 cells to study cancer cell migration and anti-cancer drug screening. Using this device, we achieved high cell viability (over 90%) and a stable analysis of the migration ability of cancer cells. We observed that the density of the cancer cells determined the probability of the occurrence of metastatic cells and that the induction of normal cells affected the metastatic velocity of each cancer cell. We verified that the increase in the migration ability of

SUBMITTER: Mi S 

PROVIDER: S-EPMC5071858 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature