Widespread occurrence of lysine methylation in Plasmodium falciparum proteins at asexual blood stages.

ABSTRACT: Post-transcriptional and post-translational modifications play a major role in Plasmodium life cycle regulation. Lysine methylation of histone proteins is well documented in several organisms, however in recent years lysine methylation of proteins outside histone code is emerging out as an important post-translational modification (PTM). In the present study we have performed global analysis of lysine methylation of proteins in asexual blood stages of Plasmodium falciparum development. We immunoprecipitated stage specific Plasmodium lysates using anti-methyl lysine specific antibodies that immunostained the asexual blood stage parasites. Using liquid chromatography and tandem mass spectrometry analysis, 570 lysine methylated proteins at three different blood stages were identified. Analysi

SUBMITTER: Kaur I 

PROVIDER: S-EPMC5071865 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature