New family of biosensors for monitoring BTX in aquatic and edaphic environments.

ABSTRACT: Benzene, toluene, ethylbenzene and xylenes (BTEX) contamination is a serious threat to public health and the environment, and therefore, there is an urgent need to detect its presence in nature. The use of whole-cell reporters is an efficient, easy-to-use and low-cost approach to detect and follow contaminants outside specialized laboratories; this is especially important in oil spills that are frequent in marine environments. The aim of this study is the construction of a bioreporter system and its comparison and validation for the specific detection of monocyclic aromatic hydrocarbons in different host bacteria and environmental samples. Our bioreporter system is based on the two component regulatory system TodS-TodT of P. putida DOT-T1E, and the P_todX promoter fused to the GF

SUBMITTER: Hernandez-Sanchez V 

PROVIDER: S-EPMC5072201 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature